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ELK Biotechnology
SKU:ES2305
ETAR Rabbit Polyclonal Antibody
ETAR Rabbit Polyclonal Antibody
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$248.00 USD
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ETAR Rabbit Polyclonal Antibody
Overview
| Product name: | ETAR rabbit pAb |
| Alternative Names: | EDNRA; ETA; ETRA; Endothelin-1 receptor; Endothelin A receptor; ET-A; ETA-R; hET-AR |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human EDNRA. AA range:378-427 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 48kD |
| GeneID(Human): | 1909 |
| Human Swiss-Prot No: | P25101 |
| Cellular localization: | Cell membrane; Multi-pass membrane protein. |
| Background: | This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009], |
| Species Reactivity: | Human;Mouse;Monkey |
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Western Blot analysis of various cells using ETAR Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunofluorescence analysis of LOVO cells, using EDNRA Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from COS7 cells, using EDNRA Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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