1
/
of
1
ELK Biotechnology
SKU:ES2264
Enolase Rabbit Polyclonal Antibody
Enolase Rabbit Polyclonal Antibody
Regular price
$248.00 USD
Regular price
Sale price
$248.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Enolase Rabbit Polyclonal Antibody
Overview
| Product name: | Enolase rabbit pAb |
| Alternative Names: | ENO2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human NSE. AA range:371-420 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 47kD |
| GeneID(Human): | 2026 |
| Human Swiss-Prot No: | P09104 |
| Cellular localization: | Cytoplasm . Cell membrane . Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. . |
| Background: | enolase 2(ENO2) Homo sapiens This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
-
Immunofluorescence analysis of Hela cell. 1,Enolase Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min. -
Western Blot analysis of various cells using Enolase Polyclonal Antibody diluted at 1:2000 -
Western Blot analysis of HepG2 cells using Enolase Polyclonal Antibody diluted at 1:2000 -
Immunofluorescence analysis of A549 cells, using NSE Antibody. The picture on the right is blocked with the synthesized peptide.
Share
