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ELK Biotechnology
SKU:ES2214
E2F-6 Rabbit Polyclonal Antibody
E2F-6 Rabbit Polyclonal Antibody
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E2F-6 Rabbit Polyclonal Antibody
Overview
| Product name: | E2F-6 rabbit pAb |
| Alternative Names: | E2F6; Transcription factor E2F6; E2F-6 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human E2F6. AA range:141-190 |
| Storage: | -20°C/1 year |
| Storage: | Rabbit |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 31kD |
| GeneID(Human): | 1876 |
| Human Swiss-Prot No: | O75461 |
| Cellular localization: | Nucleus . |
| Background: | This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using E2F-6 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western Blot analysis of HepG2 cells using E2F-6 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using E2F6 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from HepG2 cells, using E2F6 Antibody. The lane on the right is blocked with the synthesized peptide.
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