Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human DHRS4. AA range:191-240
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
32-34kD
GeneID（Human）:
10901
Human Swiss-Prot No:
Q9BTZ2
Cellular localization:
[Isoform 1]: Peroxisome . Isoform 4 is not peroxisomal. .; [Isoform 7]: Nucleus .
Background:
catalytic activity:R-CHOH-R' + NADP(+) = R-CO-R' + NADPH.,function:Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones.,miscellaneous:Inhibited by kaempferol, quercetin, genistein and myristic acid.,similarity:Belongs to the short-chain dehydrogenases/reductases (SDR) family.,subcellular location:Isoform 1 is peroxisomal, while isoform 4 is not.,subunit:Homotetramer.,tissue specificity:Isoform 1 is predominantly expressed in normal cervix (at protein level). Isoform 4 is expressed in some neoplastic cervical tissues, but not in normal cervix (at protein level). Isoforms 5 and 6 are expressed in a few neoplastic cervical tissues.,
Western Blot analysis of various cells using DHRS4 Polyclonal Antibody diluted at 1:2000
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using DHRS4 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HepG2 cells, using DHRS4 Antibody. The lane on the right is blocked with the synthesized peptide.