Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 1A1/2. AA range:71-120
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
58kD
GeneID（Human）:
1543/1544
Human Swiss-Prot No:
P04798/P05177
Cellular localization:
Endoplasmic reticulum membrane ; Peripheral membrane protein . Mitochondrion inner membrane ; Peripheral membrane protein . Microsome membrane ; Peripheral membrane protein . Cytoplasm .
Background:
This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016],
Western Blot analysis of various cells using CYP1A1/2 Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorb
Western blot analysis of lysates from COS7, RAW264.7, and HT-29 cells, using Cytochrome P450 1A1/2 Antibody. The lane on the right is blocked with the synthesized peptide.