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ELK Biotechnology
SKU:ES2001
CLK1 Rabbit Polyclonal Antibody
CLK1 Rabbit Polyclonal Antibody
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$248.00 USD
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CLK1 Rabbit Polyclonal Antibody
Overview
| Product name: | CLK1 rabbit pAb |
| Alternative Names: | CLK1; CLK; Dual specificity protein kinase CLK1; CDC-like kinase 1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CLK1. AA range:101-150 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 57kD |
| GeneID(Human): | 1195 |
| Human Swiss-Prot No: | P49759 |
| Cellular localization: | Nucleus. |
| Background: | CDC like kinase 1(CLK1) Homo sapiens This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009], |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of various cells using CLK1 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of KB lysis using CLK1 antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbe -
Immunofluorescence analysis of HUVEC cells, using CLK1 Antibody. The picture on the right is blocked with the synthesized peptide.
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