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ELK Biotechnology
SKU:ES1962
c-Fos Rabbit Polyclonal Antibody
c-Fos Rabbit Polyclonal Antibody
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c-Fos Rabbit Polyclonal Antibody
Overview
| Product name: | c-Fos rabbit pAb |
| Alternative Names: | FOS; G0S7; Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Fos. AA range:1-50 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 62kD |
| GeneID(Human): | 2353 |
| Human Swiss-Prot No: | P01100 |
| Cellular localization: | Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30. |
| Background: | The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using c-Fos Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western Blot analysis of HepG2 cells using c-Fos Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Fos Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from HepG2 cells, using Fos Antibody. The lane on the right is blocked with the synthesized peptide.
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