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ELK Biotechnology
SKU:ES1861
Catenin-α E/N Rabbit Polyclonal Antibody
Catenin-α E/N Rabbit Polyclonal Antibody
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Catenin-α E/N Rabbit Polyclonal Antibody
Overview
| Product name: | Catenin-α E/N rabbit pAb |
| Alternative Names: | CTNNA1; Catenin alpha-1; Alpha E-catenin; Cadherin-associated protein; Renal carcinoma antigen NY-REN-13; CTNNA2; CAPR; Catenin alpha-2; Alpha N-catenin; Alpha-catenin-related protein |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Catenin-alpha1. AA range:857-906 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 100kD |
| GeneID(Human): | 1495/1496 |
| Human Swiss-Prot No: | P35221/P26232 |
| Cellular localization: | [Isoform 1]: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.; [Isoform 3]: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . |
| Background: | catenin alpha 1(CTNNA1) Homo sapiens This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunofluorescence analysis of Hela cell. 1,Catenin-α E/N Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min. -
Western Blot analysis of various cells using Catenin-α E/N Polyclonal Antibody diluted at 1:500 -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Catenin-alpha1 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from HeLa cells, using Catenin-alpha1 Antibody. The lane on the right is blocked with the synthesized peptide.
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