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ELK Biotechnology
SKU:ES1839
CARD 10 Rabbit Polyclonal Antibody
CARD 10 Rabbit Polyclonal Antibody
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$248.00 USD
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CARD 10 Rabbit Polyclonal Antibody
Overview
| Product name: | CARD 10 rabbit pAb |
| Alternative Names: | CARD10; CARMA3; Caspase recruitment domain-containing protein 10; CARD-containing MAGUK protein 3; Carma 3 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CARD10. AA range:481-530 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 120kD |
| GeneID(Human): | 29775 |
| Human Swiss-Prot No: | Q9BWT7 |
| Cellular localization: | Cytoplasm . |
| Background: | The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008], |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of various cells using CARD 10 Polyclonal Antibody -
Immunofluorescence analysis of HeLa cells, using CARD10 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from HeLa and 293 cells, using CARD10 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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