BLNK; BASH; SLP65; B-cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Cytoplasmic adapter protein; Src homology 2 domain-containing leukocyte protein of 65 kDa;
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human BLNK. AA range:62-111
Storage:
-20°C/1 year
Storage:
Rabbit
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
50kD
GeneID（Human）:
29760
Human Swiss-Prot No:
Q8WV28
Cellular localization:
Cytoplasm . Cell membrane . BCR activation results in the translocation to membrane fraction.
Background:
This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012],
Western Blot analysis of various cells using BLNK Polyclonal Antibody
Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using BLNK Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from LOVO cells, treated with etoposide 25uM 24h, using BLNK Antibody. The lane on the right is blocked with the synthesized peptide.