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ELK Biotechnology
SKU:ES1728
ATP5H Rabbit Polyclonal Antibody
ATP5H Rabbit Polyclonal Antibody
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ATP5H Rabbit Polyclonal Antibody
Overview
| Product name: | ATP5H rabbit pAb |
| Alternative Names: | ATP5H; My032; ATP synthase subunit d; mitochondrial; ATPase subunit d |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ATP5H. AA range:111-160 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 26kD |
| GeneID(Human): | 10476 |
| Human Swiss-Prot No: | O75947 |
| Cellular localization: | Mitochondrion. Mitochondrion inner membrane. |
| Background: | Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using ATP5H Polyclonal Antibody diluted at 1:2000 -
Western blot analysis of lysates from HepG2 and Jurkat cells, using ATP5H Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from HepG2 cells using ATP5H antibody. -
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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