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ELK Biotechnology
SKU:ES1677
APHC Rabbit Polyclonal Antibody
APHC Rabbit Polyclonal Antibody
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$248.00 USD
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APHC Rabbit Polyclonal Antibody
Overview
| Product name: | APHC rabbit pAb |
| Alternative Names: | ACER3; APHC; PHCA; Alkaline ceramidase 3; AlkCDase 3; Alkaline CDase 3; Alkaline dihydroceramidase SB89; Alkaline phytoceramidase; aPHC |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PHCA. AA range:171-220 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 35kD |
| GeneID(Human): | 55331 |
| Human Swiss-Prot No: | Q9NUN7 |
| Cellular localization: | Endoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . |
| Background: | enzyme regulation:Activated by Ca(2+) and inhibited by Zn(2+).,function:Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.,similarity:Belongs to the alkaline ceramidase family.,tissue specificity:Ubiquitously expressed. Highest expression in placenta., |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of various cells using APHC Polyclonal Antibody -
Immunofluorescence analysis of MCF7 cells, using PHCA Antibody. The picture on the right is blocked with the synthesized peptide. -
Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using PHCA Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from COLO cells, using PHCA Antibody. The lane on the right is blocked with the synthesized peptide.
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