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ELK Biotechnology
SKU:ES1644
ALX3 Rabbit Polyclonal Antibody
ALX3 Rabbit Polyclonal Antibody
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$248.00 USD
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ALX3 Rabbit Polyclonal Antibody
Overview
| Product name: | ALX3 rabbit pAb |
| Alternative Names: | ALX3; Homeobox protein aristaless-like 3; Proline-rich transcription factor ALX3 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ALX3. AA range:171-220 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 36kD |
| GeneID(Human): | 257 |
| Human Swiss-Prot No: | O95076 |
| Cellular localization: | Nucleus . |
| Background: | This gene encodes a nuclear protein with a homeobox DNA-binding domain that functions as a transcriptional regulator involved in cell-type differentiation and development. Preferential methylation of this gene's promoter is associated with advanced-stage neuroblastoma tumors. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using ALX3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunofluorescence analysis of HeLa cells, using ALX3 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from 293 cells, using ALX3 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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