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ELK Biotechnology
SKU:ES1612
AHA-1 Rabbit Polyclonal Antibody
AHA-1 Rabbit Polyclonal Antibody
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AHA-1 Rabbit Polyclonal Antibody
Overview
| Product name: | AHA-1 rabbit pAb |
| Alternative Names: | AHSA1; C14orf3; HSPC322; Activator of 90 kDa heat shock protein ATPase homolog 1; AHA1; p38 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300;ELISA 1:5000-20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human AHSA1. AA range:161-210 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 38kD |
| GeneID(Human): | 10598 |
| Human Swiss-Prot No: | O95433 |
| Cellular localization: | Cytoplasm, cytosol . Endoplasmic reticulum . May transiently interact with the endoplasmic reticulum. |
| Background: | function:Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.,induction:By heat shock and treatment with the HSP90 inhibitor 17-demethoxygeldanamycin (17AAG).,similarity:Belongs to the AHA1 family.,subcellular location:May transiently interact with the endoplasmic reticulum.,subunit:Interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatitis virus glycoprotein (VSV G). Interacts with GCH1.,tissue specificity:Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta., |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of various cells using AHA-1 Polyclonal Antibody diluted at 1:1000 -
Western blot analysis of lysates from HT-29, A549, and K562 cells, using AHSA1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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