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ELK Biotechnology
SKU:ES1609
AF-10 Rabbit Polyclonal Antibody
AF-10 Rabbit Polyclonal Antibody
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AF-10 Rabbit Polyclonal Antibody
Overview
| Product name: | AF-10 rabbit pAb |
| Alternative Names: | MLLT10; AF10; Protein AF-10; ALL1-fused gene from chromosome 10 protein |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human AF10. AA range:211-260 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 109kD |
| GeneID(Human): | 8028 |
| Human Swiss-Prot No: | P55197 |
| Cellular localization: | Nucleus . |
| Background: | myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10(MLLT10) Homo sapiens This gene encodes a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010], |
| Species Reactivity: | Human;Mouse |
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Immunofluorescence analysis of Hela cell. 1,AF-10 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). COX IV Monoclonal Antibody(6C8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1 -
Western Blot analysis of various cells using AF-10 Polyclonal Antibody diluted at 1:1000 -
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor -
Western blot analysis of lysates from COLO205 and Jurkat cells, using AF10 Antibody. The lane on the right is blocked with the synthesized peptide.
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