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ELK Biotechnology
SKU:ES1591
Actin α3 Rabbit Polyclonal Antibody
Actin α3 Rabbit Polyclonal Antibody
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$248.00 USD
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Actin α3 Rabbit Polyclonal Antibody
Overview
| Product name: | Actin α3 rabbit pAb |
| Alternative Names: | ACTG2; ACTA3; ACTL3; ACTSG; Actin; gamma-enteric smooth muscle; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Actin-gamma2. AA range:1-50 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 45kD |
| GeneID(Human): | 72 |
| Human Swiss-Prot No: | P63267 |
| Cellular localization: | Cytoplasm, cytoskeleton. |
| Background: | Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using Actin α3 Polyclonal Antibody diluted at 1:2000 -
Western Blot analysis of COLO205 cells using Actin α3 Polyclonal Antibody diluted at 1:2000 -
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Actin-gamma2 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from COLO205 cells, using Actin-gamma2 Antibody. The lane on the right is blocked with the synthesized peptide.
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