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ELK Biotechnology
SKU:ES1585
ACOT8 Rabbit Polyclonal Antibody
ACOT8 Rabbit Polyclonal Antibody
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ACOT8 Rabbit Polyclonal Antibody
Overview
| Product name: | ACOT8 rabbit pAb |
| Alternative Names: | ACOT8; ACTEIII; PTE1; PTE2; Acyl-coenzyme A thioesterase 8; Acyl-CoA thioesterase 8; Choloyl-coenzyme A thioesterase; HIV-Nef-associated acyl-CoA thioesterase; PTE-2; Peroxisomal acyl-coenzyme A thioester hydrolase 1; PTE-1; Peroxisomal lon |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ACOT8. AA range:131-180 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 36kD |
| GeneID(Human): | 10005 |
| Human Swiss-Prot No: | O14734 |
| Cellular localization: | Peroxisome matrix . Predominantly localized in the peroxisome but a localization to the cytosol cannot be excluded. . |
| Background: | The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010], |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of various cells using ACOT8 Polyclonal Antibody -
Western Blot analysis of HuvEc cells using ACOT8 Polyclonal Antibody -
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Immunofluorescence analysis of A549 cells, using ACOT8 Antibody. The picture on the right is blocked with the synthesized peptide.
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