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ELK Biotechnology
SKU:ES1584
ACOT4 Rabbit Polyclonal Antibody
ACOT4 Rabbit Polyclonal Antibody
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$248.00 USD
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ACOT4 Rabbit Polyclonal Antibody
Overview
| Product name: | ACOT4 rabbit pAb |
| Alternative Names: | ACOT4; PTE2B; PTEIB; Acyl-coenzyme A thioesterase 4; Acyl-CoA thioesterase 4; PTE-2b; Peroxisomal acyl coenzyme A thioester hydrolase Ib; Peroxisomal long-chain acyl-CoA thioesterase Ib; PTE-Ib |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ACOT4. AA range:361-410 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 48kD |
| GeneID(Human): | 122970 |
| Human Swiss-Prot No: | Q8N9L9 |
| Cellular localization: | Peroxisome . |
| Background: | catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.,similarity:Belongs to the C/M/P thioester hydrolase family., |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of various cells using ACOT4 Polyclonal Antibody diluted at 1:1000 -
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Immunofluorescence analysis of MCF7 cells, using ACOT4 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from HeLa and COLO cells, using ACOT4 Antibody. The lane on the right is blocked with the synthesized peptide.
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