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ELK Biotechnology
SKU:ES1544
14-3-3 θ Rabbit Polyclonal Antibody
14-3-3 θ Rabbit Polyclonal Antibody
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14-3-3 θ Rabbit Polyclonal Antibody
Overview
| Product name: | 14-3-3 θ rabbit pAb |
| Alternative Names: | YWHAQ; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human 14-3-3 thet. AA range:41-90 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 28kD |
| GeneID(Human): | 10971 |
| Human Swiss-Prot No: | P27348 |
| Cellular localization: | Cytoplasm. In neurons, axonally transported to the nerve terminals. |
| Background: | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using 14-3-3 θ Polyclonal Antibody diluted at 1:1000 -
Western blot analysis of lysates from HUVEC and HeLa cells, using 14-3-3 thet Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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