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ELK Biotechnology
SKU:ES1523
DARPP-32 (phospho Thr34) Rabbit Polyclonal Antibody
DARPP-32 (phospho Thr34) Rabbit Polyclonal Antibody
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DARPP-32 (phospho Thr34) Rabbit Polyclonal Antibody
Overview
| Product name: | DARPP-32 (phospho Thr34) rabbit pAb |
| Alternative Names: | PPP1R1B; DARPP32; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr34. AA range:18-67 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 35kD |
| GeneID(Human): | 84152 |
| Human Swiss-Prot No: | Q9UD71 |
| Cellular localization: | Cytoplasm. |
| Background: | This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using Phospho-DARPP-32 (T34) Polyclonal Antibody diluted at 1:1000 -
Western Blot analysis of RAT-musle cells using Phospho-DARPP-32 (T34) Polyclonal Antibody diluted at 1:1000 -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DARPP-32 (Phospho-Thr34) Antibody -
Immunofluorescence analysis of HepG2 cells, using DARPP-32 (Phospho-Thr34) Antibody. The picture on the right is blocked with the phospho peptide.
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