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ELK Biotechnology
SKU:ES1507
HSP90β (phospho Ser254) Rabbit Polyclonal Antibody
HSP90β (phospho Ser254) Rabbit Polyclonal Antibody
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HSP90β (phospho Ser254) Rabbit Polyclonal Antibody
Overview
| Product name: | HSP90β (phospho Ser254) rabbit pAb |
| Alternative Names: | HSP90AB1; HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254. AA range:226-275 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 90kD |
| GeneID(Human): | 3326 |
| Human Swiss-Prot No: | P08238 |
| Cellular localization: | Cytoplasm . Melanosome . Nucleus . Secreted . Cell membrane . Dynein axonemal particle . Cell surface . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Translocates with BIRC2 from the nucleus to the cytoplasm during differentiation (PubMed:18239673). Secreted when associated with TGFB1 processed form (LAP) (PubMed:20599762). . |
| Background: | This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012], |
| Species Reactivity: | Human;Mouse;Rat;Monkey |
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Western Blot analysis of various cells using Phospho-HSP90β (S254) Polyclonal Antibody -
Western Blot analysis of HepG2 cells using Phospho-HSP90β (S254) Polyclonal Antibody -
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HSP90B (Phospho-Ser254) Antibody
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