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ELK Biotechnology
SKU:ES1470
eEF2K (phospho Ser366) Rabbit Polyclonal Antibody
eEF2K (phospho Ser366) Rabbit Polyclonal Antibody
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eEF2K (phospho Ser366) Rabbit Polyclonal Antibody
Overview
| Product name: | eEF2K (phospho Ser366) rabbit pAb |
| Alternative Names: | EEF2K; Eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human eEF2K around the phosphorylation site of Ser366. AA range:331-380 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 105kD |
| GeneID(Human): | 29904 |
| Human Swiss-Prot No: | O00418 |
| Cellular localization: | cytoplasm,cytosol,postsynaptic density, |
| Background: | This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Rat |
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Western Blot analysis of various cells using Phospho-eEF2K (S366) Polyclonal Antibody diluted at 1:1000 -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eEF2K (Phospho-Ser366) Antibody -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2K (Phospho-Ser366) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from HeLa cells treated with serum 10% 15', using eEF2K (Phospho-Ser366) Antibody. The lane on the right is blocked with the phospho peptide.
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