C/EBP α (phospho Ser21) Rabbit Polyclonal Antibody

Name: C/EBP α (phospho Ser21) Rabbit Polyclonal Antibody
Brand: ELK Biotechnology
SKU: ES1468
Price: 248.0 USD
Availability: InStock

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C/EBP α (phospho Ser21) Rabbit Polyclonal Antibody

Overview

Product name:	C/EBP α (phospho Ser21) rabbit pAb
Alternative Names:	CEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha
Applications:	WB;IHC;IF;ELISA
Recommended Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human C/EBP-alpha around the phosphorylation site of Ser21. AA range:6-55
Storage:	Rabbit
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	42kd
GeneID（Human）:	1050
Human Swiss-Prot No:	P49715
Cellular localization:	Nucleus .; [Isoform 4]: Nucleus, nucleolus .
Background:	This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013],
Species Reactivity:	Human;Mouse;Rat

Western blot analysis of lysates from Mouse-liver, Rat-liver, Hela cells treated with 0.01U/ml 30min, using C/EBP α (phospho Ser21) Polyclonal Antibody. Primary Antibody was diluted at 1:1000 4° over night,secondary antibody(Immunoway cat:RS23710)was dilu
Western Blot analysis of various cells using Phospho-C/EBP α (S21) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbe
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C/EBP-alpha (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.

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C/EBP α (phospho Ser21) Rabbit Polyclonal Antibody

C/EBP α (phospho Ser21) Rabbit Polyclonal Antibody

Overview