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ELK Biotechnology
SKU:ES1461
Synaptotagmin 1/2 (phospho Thr202/199) Rabbit Polyclonal Antibody
Synaptotagmin 1/2 (phospho Thr202/199) Rabbit Polyclonal Antibody
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Synaptotagmin 1/2 (phospho Thr202/199) Rabbit Polyclonal Antibody
Overview
| Product name: | Synaptotagmin 1/2 (phospho Thr202/199) rabbit pAb |
| Alternative Names: | SYT1; SVP65; SYT; Synaptotagmin-1; Synaptotagmin I; SytI; p65; SYT2; Synaptotagmin-2; Synaptotagmin II; SytII |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Synaptotagmin around the phosphorylation site of Thr202. AA range:176-225 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 60kD |
| GeneID(Human): | 6857/127833 |
| Human Swiss-Prot No: | P21579/Q8N9I0 |
| Cellular localization: | Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane ; Single-pass membrane protein . Cytoplasm . |
| Background: | The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using Phospho-Synaptotagmin 1/2 (T202/199) Polyclonal Antibody -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Synaptotagmin (Phospho-Thr202) Antibody -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Synaptotagmin (Phospho-Thr202) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from 293 cells treated with Forskolin 40nM 30', using Synaptotagmin (Phospho-Thr202) Antibody. The lane on the left is blocked with the phospho peptide.
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