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ELK Biotechnology

ES1443

Raf-1 (phospho Ser338) Rabbit Polyclonal Antibody

Raf-1 (phospho Ser338) Rabbit Polyclonal Antibody

$248.00 USD
$248.00 USD
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Raf-1 (phospho Ser338) Rabbit Polyclonal Antibody

Overview

Product name: Raf-1 (phospho Ser338) rabbit pAb
Alternative Names: RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Applications: WB;ELISA;IHC
Recommended Dilutions: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen: The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser338. AA range:305-354
Storage: Rabbit
Storage: -20°C/1 year
Clonality: Polyclonal
Isotype: IgG
Concentration: 1 mg/ml
Observed Band: 70kD
GeneID（Human）: 5894
Human Swiss-Prot No: P04049
Cellular localization: Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.
Background: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008],
Species Reactivity: Human;Mouse;Rat
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