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ELK Biotechnology
SKU:ES1332
HSP27 (phospho Ser82) Rabbit Polyclonal Antibody
HSP27 (phospho Ser82) Rabbit Polyclonal Antibody
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HSP27 (phospho Ser82) Rabbit Polyclonal Antibody
Overview
| Product name: | HSP27 (phospho Ser82) rabbit pAb |
| Alternative Names: | HSPB1; HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HSP27 around the phosphorylation site of Ser82. AA range:48-97 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 27kD |
| GeneID(Human): | 3315 |
| Human Swiss-Prot No: | P04792 |
| Cellular localization: | Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. . |
| Background: | The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using Phospho-HSP27 (S82) Polyclonal Antibody diluted at 1:1000 -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HSP27 (Phospho-Ser82) Antibody -
Western blot analysis of lysates from HUVEC cells treated with TNF 20ng/ml 30', using HSP27 (Phospho-Ser82) Antibody. The lane on the right is blocked with the phospho peptide. -
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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