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ELK Biotechnology
SKU:ES1328
Histone H3 (phospho Ser28) Rabbit Polyclonal Antibody
Histone H3 (phospho Ser28) Rabbit Polyclonal Antibody
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Histone H3 (phospho Ser28) Rabbit Polyclonal Antibody
Overview
| Product name: | Histone H3 (phospho Ser28) rabbit pAb |
| Alternative Names: | HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3 |
| Applications: | WB;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Histone H3 around the phosphorylation site of Ser28. AA range:13-62 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 20kD |
| GeneID(Human): | 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968 |
| Human Swiss-Prot No: | P68431/Q71DI3/P84243 |
| Cellular localization: | Nucleus. Chromosome. |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using Phospho-Histone H3 (S28) Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western Blot analysis of 293T cells using Phospho-Histone H3 (S28) Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of lysates from HUVEC cells treated with Serum 20% 30', using Histone H3 (Phospho-Ser28) Antibody. The lane on the right is blocked with the phospho peptide.
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