Histone H2A.X (phospho Ser139) Rabbit Polyclonal Antibody

Name: Histone H2A.X (phospho Ser139) Rabbit Polyclonal Antibody
Brand: ELK Biotechnology
SKU: ES1326
Price: 248.0 USD
Availability: InStock

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Histone H2A.X (phospho Ser139) Rabbit Polyclonal Antibody

Overview

Product name:	Histone H2A.X (phospho Ser139) rabbit pAb
Alternative Names:	H2AFX; H2AX; Histone H2A.x; H2a/x
Applications:	WB;IHC;IF;ELISA
Recommended Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human Histone H2A.X around the phosphorylation site of Ser139. AA range:94-143
Storage:	Rabbit
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	15 19kD
GeneID（Human）:	3014
Human Swiss-Prot No:	P16104
Cellular localization:	Nucleus . Chromosome .
Background:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015],
Species Reactivity:	Human;Mouse;Rat;Hamster

Western blot analysis of lysates from 1) 4T1, 2) 293 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (Immunoway
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary ant
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody wa

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Histone H2A.X (phospho Ser139) Rabbit Polyclonal Antibody

Histone H2A.X (phospho Ser139) Rabbit Polyclonal Antibody

Overview