GSK3α/β (phospho Tyr279/216) Rabbit Polyclonal Antibody

Name: GSK3α/β (phospho Tyr279/216) Rabbit Polyclonal Antibody
Brand: ELK Biotechnology
SKU: ES1321
Price: 248.0 USD
Availability: InStock

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GSK3α/β (phospho Tyr279/216) Rabbit Polyclonal Antibody

Overview

Product name:	GSK3α/β (phospho Tyr279/216) rabbit pAb
Alternative Names:	GSK3A; Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
Applications:	IF;WB;IHC;ELISA
Recommended Dilutions:	IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human GSK3 alpha/beta around the phosphorylation site of Tyr279/216. AA range:246-295
Storage:	Rabbit
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	51,46kD
GeneID（Human）:	2931
Human Swiss-Prot No:	P49840/P49841
Cellular localization:	mitochondrion,cytosol,beta-catenin destruction complex,postsynapse,
Background:	glycogen synthase kinase 3 alpha(GSK3A) Homo sapiens This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011],
Species Reactivity:	Human;Mouse;Rat

Immunofluorescence analysis of Hela cell. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Immunofluorescence analysis of mouse-liver tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Immunofluorescence analysis of mouse-liver tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and

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GSK3α/β (phospho Tyr279/216) Rabbit Polyclonal Antibody

GSK3α/β (phospho Tyr279/216) Rabbit Polyclonal Antibody

Overview