DARPP-32 (phospho Thr75) Rabbit Polyclonal Antibody

Name: DARPP-32 (phospho Thr75) Rabbit Polyclonal Antibody
Brand: ELK Biotechnology
SKU: ES1297
Price: 248.0 USD
Availability: InStock

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DARPP-32 (phospho Thr75) Rabbit Polyclonal Antibody

Overview

Product name:	DARPP-32 (phospho Thr75) rabbit pAb
Alternative Names:	PPP1R1B; DARPP32; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein
Applications:	WB;IHC;IF;ELISA
Recommended Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr75. AA range:41-90
Storage:	Rabbit
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	32kD
GeneID（Human）:	84152
Human Swiss-Prot No:	Q9UD71
Cellular localization:	Cytoplasm.
Background:	This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
Species Reactivity:	Human;Mouse;Rat;Monkey

Western Blot analysis of cell lysis treated or untreated by LPS 100ng/mL 30min, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
Western Blot analysis of various cells using Phospho-DARPP-32 (T75) Polyclonal Antibody diluted at 1:500
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor

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DARPP-32 (phospho Thr75) Rabbit Polyclonal Antibody

DARPP-32 (phospho Thr75) Rabbit Polyclonal Antibody

Overview