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ELK Biotechnology
SKU:ES1260
AP-1 (phospho Ser63) Rabbit Polyclonal Antibody
AP-1 (phospho Ser63) Rabbit Polyclonal Antibody
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AP-1 (phospho Ser63) Rabbit Polyclonal Antibody
Overview
| Product name: | AP-1 (phospho Ser63) rabbit pAb |
| Alternative Names: | JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 |
| Applications: | IF;WB;IHC;ELISA |
| Recommended Dilutions: | IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human c-Jun around the phosphorylation site of Ser63. AA range:31-80 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 39-42kD |
| GeneID(Human): | 3725 |
| Human Swiss-Prot No: | P05412 |
| Cellular localization: | Nucleus. |
| Background: | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunofluorescence analysis of mouse-testis tissue. 1,AP-1 (phospho Ser63) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture -
Immunofluorescence analysis of mouse-testis tissue. 1,AP-1 (phospho Ser63) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture -
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,AP-1 (phospho Ser63) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at -
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,AP-1 (phospho Ser63) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at
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