Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized Acetyl-peptide derived from human TUBA1B around the Acetylation site of Lys352. AA range:311-360
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
50kD
GeneID（Human）:
7846
Human Swiss-Prot No:
Q71U36
Cellular localization:
Cytoplasm, cytoskeleton.
Background:
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.
Western Blot analysis of K562, A549, Jurkat cells using Acetyl-Tubulin α (K352) Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western Blot analysis of K562 cells using Acetyl-Tubulin α (K352) Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western blot analysis of lysate from K562 cells, using TUBA1B (Acetyl-Lys352) Antibody.