Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized Acetyl-peptide derived from human EEF1A around the Acetylation site of Lys41. AA range:1-50
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
50kD
GeneID（Human）:
1915
Human Swiss-Prot No:
P68104
Cellular localization:
Cytoplasm . Nucleus . Nucleus, nucleolus . Cell membrane . Colocalizes with DLC1 at actin-rich regions in the cell periphery (PubMed:19158340). Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens (PubMed:8650580). Localization at the cell membrane depends on EEF1A1 phosphorylation status and the presence of PPP1R16B (PubMed:26497934). .
Background:
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008],
Western Blot analysis of A549 cells using Acetyl-EF-1 α1/2 (K41) Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western Blot analysis of A549 cells using Acetyl-EF-1 α1/2 (K41) Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western blot analysis of lysate from A549 cells, using EEF1A-pan (Acetyl-Lys41) Antibody.