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ELK Biotechnology
SKU:ES1092
Histone H4 (Acetyl Lys8) Rabbit Polyclonal Antibody
Histone H4 (Acetyl Lys8) Rabbit Polyclonal Antibody
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Histone H4 (Acetyl Lys8) Rabbit Polyclonal Antibody
Overview
| Product name: | Histone H4 (Acetyl Lys8) rabbit pAb |
| Alternative Names: | HIST1H4A; H4/A; H4FA; HIST1H4B; H4/I; H4FI; HIST1H4C; H4/G; H4FG; HIST1H4D; H4/B; H4FB; HIST1H4E; H4/J; H4FJ; HIST1H4F; H4/C; H4FC; HIST1H4H; H4/H; H4FH; HIST1H4I; H4/M; H4FM; HIST1H4J; H4/E; H4FE; HIST1H4K; H4/D; H4FD; HIST1H4L; H4/K; H4FK;H4K8AC |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys8. AA range:1-50 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 11kD |
| GeneID(Human): | 121504/554313/8294/8359/8360/8361/8362/8363/8364/8365/8366/8367/8368/8370 |
| Human Swiss-Prot No: | P62805 |
| Cellular localization: | Nucleus. Chromosome. |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015], |
| Species Reactivity: | Human;Mouse;Rat;Monkey |
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Immunofluorescence analysis of Hela cell. 1,Histone H4 (Acetyl Lys8) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). HER2 Monoclonal Antibody(11H9)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 -
Western Blot analysis of various cells using Acetyl-Histone H4 (K8) Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 -
Western Blot analysis of KB cells using Acetyl-Histone H4 (K8) Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 -
Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys8) Antibody. The picture on the right is blocked with the synthesized peptide.
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