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ELK Biotechnology
SKU:ES1087
Histone H2B (Acetyl Lys5) Rabbit Polyclonal Antibody
Histone H2B (Acetyl Lys5) Rabbit Polyclonal Antibody
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Histone H2B (Acetyl Lys5) Rabbit Polyclonal Antibody
Overview
| Product name: | Histone H2B (Acetyl Lys5) rabbit pAb |
| Alternative Names: | H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s;H2BK5AC |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys5. AA range:1-50 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 18kD |
| GeneID(Human): | 54145 |
| Human Swiss-Prot No: | P57053 |
| Cellular localization: | Nucleus. Chromosome. |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of various cells using Acetyl-Histone H2B (K5) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 -
Western Blot analysis of 3T3 cells using Acetyl-Histone H2B (K5) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 -
Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys5) Antibody. The picture on the right is blocked with the synthesized peptide. -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys5) Antibody. The picture on the right is blocked with the synthesized peptide.
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