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ELK Biotechnology

ES1076

His-tag Rabbit Polyclonal Antibody

His-tag Rabbit Polyclonal Antibody

$248.00 USD
$248.00 USD
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His-tag Rabbit Polyclonal Antibody

Overview

Product name: His-tag rabbit pAb
Alternative Names: 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Applications: WB;ELISA
Recommended Dilutions: Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen: 6X His synthetic peptide conjugated to KLH.
Storage: Rabbit
Storage: -20°C/1 year
Clonality: Polyclonal
Isotype: IgG
Concentration: 1 mg/ml
Observed Band: 15kD
Background: The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.
Species Reactivity: Species independent
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