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ELK Biotechnology
SKU:EA116
Histone H2A.X(Acetyl Lys5) Rabbit Polyclonal Antibody
Histone H2A.X(Acetyl Lys5) Rabbit Polyclonal Antibody
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Histone H2A.X(Acetyl Lys5) Rabbit Polyclonal Antibody
Overview
| Product name: | Histone H2A.X(Acetyl Lys5) Rabbit pAb |
| Reactivity: | Human, Rat, Mouse |
| Alternative Names: | H2A histone family member X antidoby, H2AFX antibody, H2AX Histoneh antibody, Histone H2AX antibody |
| Source: | Rabbit |
| Applications: | WB, IHC |
| Species: | Human |
| Recommended dilutions: | WB 1:1,000-2,000 IHC 1:200-500 |
| Immunogen: | Synthetic Peptide |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Polyclonal |
| Isotype: | Antiserum |
| Concentration: | 1mg/ml |
| Observed band: | 15kDa |
| GeneID(Human): | 3014 |
| Human Swiss-Prot No: | P16104 |
| Cellular localization: | Nucleus,Chromosome |
| Background: | Histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. |
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Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +), using Acetyl-Histone H2A.X (Lys5) Rabbit pAb (upper) EA116 1:2,000 and β-actin Mouse mAb (lower) EM1031 1:5,000. -
Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using Histone H2A.X (Acetyl Lys5)(E116)Rabbit pAb diluted at 1:500.
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