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ELK Biotechnology
SKU:EA064
Histone H3(Phospho Thr32 ) Rabbit Polyclonal Antibody
Histone H3(Phospho Thr32 ) Rabbit Polyclonal Antibody
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$248.00 USD
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Histone H3(Phospho Thr32 ) Rabbit Polyclonal Antibody
Overview
| Product name: | Histone H3(Phospho Thr32 ) Rabbit pAb |
| Reactivity: | Human, Rat, Mouse |
| Alternative Names: | H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H7a antibody |
| Source: | Rabbit |
| Applications: | WB, IHC |
| Species: | Human |
| Recommended dilutions: | WB 1:1,000-2,000 IHC 1:200-500 |
| Immunogen: | Synthetic Peptide |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1mg/ml |
| Observed band: | 15-17kDa |
| GeneID(Human): | 8290 |
| Human Swiss-Prot No: | P68431 |
| Cellular localization: | Nuclear |
| Background: | Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. |
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Western blot analysis of extracts from Hela cells, untreated (-) or treated with Nocodazole (1μg/ml, 24 hr; +), using Phospho-Histone H3 (Thr32) Rabbit pAb (upper) EA064 1:2000 and Histone H3 (1G1) Mouse mAb (lower) EM1037 1:2000. -
Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using Histone H3(Phospho Thr32)(EA064)Rabbit pAb diluted at 1:500.
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