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ELK Biotechnology
SKU:EM1159
Vimentin Mouse Monoclonal Antibody
Vimentin Mouse Monoclonal Antibody
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$248.00 USD
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Vimentin Mouse Monoclonal Antibody
Overview
| Product name: | Vimentin Mouse mAb |
| Reactivity: | Human, Rat, Mouse |
| Alternative Names: | FLJ36605 antibody, VIM antibody, VIME antibody |
| Source: | Mouse |
| Applications: | WB, IHC, IF |
| Species: | Human |
| Recommended dilutions: | WB 1:1,000-3,000 IF 1:100-200 IHC 1:200-500 |
| Immunogen: | Recombinant Protein |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | 50-57kDa |
| GeneID(Human): | 7431 |
| Human Swiss-Prot No: | P08670 |
| Cellular localization: | Cytoplasm |
| Background: | The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules.Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. |
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Western blot analysis of 1) Hela, 2)Mouse Brain Tissue , 3) Rat Brain tissue with EM1159 diluted at 1:2,000. -
IF analysis of Hela with EM1159(Left) and DAPI (Right) diluted at 1:100. -
Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Vimentin (EM1159)Mouse mAb diluted at 1:500. -
Western blot analysis of extracts from MCF-7 using Vimentin diluted at 1:3,000
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