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ELK Biotechnology
SKU:EM1136
eIF4A1 Mouse Monoclonal Antibody
eIF4A1 Mouse Monoclonal Antibody
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$248.00 USD
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eIF4A1 Mouse Monoclonal Antibody
Overview
| Product name: | eIF4A1 Mouse mAb |
| Reactivity: | Human, Rat, Mouse |
| Alternative Names: | DDX2, eIF 4A I, Eukaryotic initiation factor 4A-1, ATP-dependent RNA helicase eIF4A-1,DDX2A |
| Source: | Mouse |
| Applications: | WB, IHC, IF |
| Species: | Human |
| Recommended dilutions: | WB 1:1,000-3,000 IF 1:100-200 IHC 1:100-200 |
| Immunogen: | Synthetic Peptide |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | 48kDa |
| GeneID(Human): | 1973 |
| Human Swiss-Prot No: | P60842 |
| Cellular localization: | Cytosol,Extracellular region or secreted |
| Background: | The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA – this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression. |
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Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with EM1136 diluted at 1:2,000. -
IF analysis of Hela with EM1136(Left) and DAPI (Right) diluted at 1:100. -
Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using eIF4A1(EM1136)Mouse mAb diluted at 1:200.
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