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ELK Biotechnology
SKU:EM1095
Cytokeratin 18 Mouse Monoclonal Antibody
Cytokeratin 18 Mouse Monoclonal Antibody
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$248.00 USD
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Cytokeratin 18 Mouse Monoclonal Antibody
Overview
| Product name: | Cytokeratin 18 Mouse mAb |
| Reactivity: | Human, Rat, Mouse |
| Alternative Names: | Cell proliferation inducing gene 46 protein, CK 18, CYK 18, K 18, Keratin 18, Ketatin D, KRT18 |
| Source: | Mouse |
| Applications: | WB, IHC, IF |
| Species: | Human |
| Recommended dilutions: | WB 1:1,000-3,000 IF 1:100-200 IHC 1:200-500 |
| Immunogen: | Recombinant Protein |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | 46kDa |
| GeneID(Human): | 3875 |
| Human Swiss-Prot No: | P05783 |
| Cellular localization: | Cytoplasm, Intermediate filament, Keratin, Nucleus |
| Background: | Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. |
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Western blot analysis of 1) HepG2, 2) Hela, 3) Mouse Liver tissue, 4) C2C12, 5) Rat Heart tissue, 6) Mouse Skeletal Muscle tissue with Cytokeratin 18 Mouse MAb (1B3) diluted at 1:2000. -
IF analysis of Hela with EM1095(Left) and DAPI (Right) diluted at 1:100. -
Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Cytokeratin 18 (EM1095)Mouse mAb diluted at 1:500.
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