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ELK Biotechnology
SKU:EM1077
Caspase 9 Mouse Monoclonal Antibody
Caspase 9 Mouse Monoclonal Antibody
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$248.00 USD
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Caspase 9 Mouse Monoclonal Antibody
Overview
| Product name: | Caspase 9 Mouse mAb |
| Reactivity: | Human, Rat, Mouse |
| Alternative Names: | APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody |
| Source: | Mouse |
| Applications: | WB, IP, IF |
| Species: | Human |
| Recommended dilutions: | WB 1:1,000-5,000 IP 1:200 IF 1:100-200 |
| Immunogen: | Synthetic Peptide |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | 47(51)kDa |
| GeneID(Human): | 842 |
| Human Swiss-Prot No: | P55211 |
| Cellular localization: | Cytosol,Mitochondrion,Nucleus |
| Background: | Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). |
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Western blot analysis of Hela with Caspase 9 Mouse mAb diluted at 1)1:2,000 2)1:5,000 -
Western blot analysis of 1)Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 Mouse mAb diluted at 1:3,000 -
1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1077 1:1,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000 -
IF analysis of Hela with EM1077(Left) and DAPI (Right) diluted at 1:100.
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