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ELK Biotechnology
SKU:EM1054
CK7 Mouse Monoclonal Antibody
CK7 Mouse Monoclonal Antibody
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$248.00 USD
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CK7 Mouse Monoclonal Antibody
Overview
| Product name: | CK7 Mouse mAb |
| Reactivity: | Human, Rat, Mouse |
| Alternative Names: | Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7 |
| Source: | Mouse |
| Applications: | WB, IHC, IP |
| Species: | Human, Rat, Mouse |
| Species: | Human |
| Recommended dilutions: | WB 1:1,000-2,000 IHC 1:200 IP 1:200 |
| Immunogen: | Synthetic Peptide |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | 54kDa |
| GeneID(Human): | 3855 |
| Human Swiss-Prot No: | P08729 |
| Cellular localization: | Cytoplasm |
| Background: | Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19. |
Precision
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
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Western blot analysis of 1) Hela, 2)Mouse Kidney, 3) Mouse Brain with CK7 mAb diluted at 1:2,000. -
IHC staining of human lung cancer tissue with CK7 mouse mAb(12D7) diluted at 1:200. -
1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1054 1:1,000 Secondary antibody: Goat anti-Mouse IgG Light chain specific(S003), 1:5,000 -
Western blot analysis of extracts from MCF-7 (Lane 1), Mouse Kidney (Lane 2), Rat Kidney (Lane 3), using CK7 diluted at 1:2,000.
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