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ELK Biotechnology

SKU:EM1054

CK7 Mouse Monoclonal Antibody

CK7 Mouse Monoclonal Antibody

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CK7 Mouse Monoclonal Antibody

Overview

Product name: CK7 Mouse mAb
Reactivity: Human, Rat, Mouse
Alternative Names: Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7
Source: Mouse
Applications: WB, IHC, IP
Species: Human, Rat, Mouse
Species: Human
Recommended dilutions: WB 1:1,000-2,000 IHC 1:200 IP 1:200
Immunogen: Synthetic Peptide
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/ml
Observed band: 54kDa
GeneID(Human): 3855
Human Swiss-Prot No: P08729
Cellular localization: Cytoplasm
Background: Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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