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ELK Biotechnology
SKU:EM1052
p53 Mouse Monoclonal Antibody
p53 Mouse Monoclonal Antibody
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$248.00 USD
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p53 Mouse Monoclonal Antibody
Overview
| Product name: | p53 Mouse mAb |
| Reactivity: | Human |
| Alternative Names: | Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53 |
| Source: | Mouse |
| Applications: | WB, IHC, IF |
| Species: | Human |
| Species: | Human |
| Recommended dilutions: | WB 1:2,000 IHC 1:200 IF 1:100-200 |
| Immunogen: | Synthetic Peptide |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | 53kDa |
| GeneID(Human): | 7157 |
| Human Swiss-Prot No: | P04637 |
| Cellular localization: | Cytoplasm; Cytoplasm. Nucleus |
| Background: | p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. |
Precision
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
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Western blot analysis of 293T, with p53 mAb diluted at 1) 1:1,000 2) 1:2,000 -
IHC staining of Human colon cancer tissue paraffin-embedded with p53 mouse mAb(6C4)diluted at 1:200. -
IF analysis of Hela with EM1052(Left) and DAPI (Right) diluted at 1:100. -
Western blot analysis of extracts from Huh7 using p53 diluted at 1:2,000.
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