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ELK Biotechnology

EM1046

CD45 Mouse Monoclonal Antibody

CD45 Mouse Monoclonal Antibody

$248.00 USD
$248.00 USD
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CD45 Mouse Monoclonal Antibody

Overview

Product name: CD45 Mouse mAb
Reactivity: Human
Alternative Names: B220, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200
Source: Mouse
Applications: WB
Species: Human
Species: Human
Recommended dilutions: WB 1:2,000 IHC 1:200
Immunogen: Synthetic Peptide
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/ml
Observed band: 147kDa
GeneID（Human）: 5788
Human Swiss-Prot No: P08575
Cellular localization: Membrane
Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45.

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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