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ELK Biotechnology

SKU:EM1037

Histone H3 Mouse Monoclonal Antibody

Histone H3 Mouse Monoclonal Antibody

Regular price $248.00 USD
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Histone H3 Mouse Monoclonal Antibody

Overview

Product name: Histone H3 Mouse mAb
Reactivity: Human, Mouse, Rat, Yeast
Alternative Names: H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
Source: Mouse
Applications: WB, IHC, IF, IP
Species: Human, Mouse, Rat, Yeast
Species: Human
Recommended dilutions: WB 1:2,000-5,000 IP 1:200 IF 1:200
Immunogen: Recombinant Protein
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/ml
Observed band: 15kDa
GeneID(Human): 8290
Human Swiss-Prot No: P68431
Cellular localization: Nucleus,Chromosome
Background: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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