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ELK Biotechnology
SKU:EM1037
Histone H3 Mouse Monoclonal Antibody
Histone H3 Mouse Monoclonal Antibody
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$248.00 USD
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Histone H3 Mouse Monoclonal Antibody
Overview
| Product name: | Histone H3 Mouse mAb |
| Reactivity: | Human, Mouse, Rat, Yeast |
| Alternative Names: | H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody |
| Source: | Mouse |
| Applications: | WB, IHC, IF, IP |
| Species: | Human, Mouse, Rat, Yeast |
| Species: | Human |
| Recommended dilutions: | WB 1:2,000-5,000 IP 1:200 IF 1:200 |
| Immunogen: | Recombinant Protein |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | 15kDa |
| GeneID(Human): | 8290 |
| Human Swiss-Prot No: | P68431 |
| Cellular localization: | Nucleus,Chromosome |
| Background: | Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. |
Precision
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
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Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue with EM1037 diluted at 1:5,000. -
IF analysis of Hela with DAPI (Left) and EM1037(Right) diluted at 1:200. -
1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1037 1:2,000 Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000 -
Western blot analysis of extracts from HeLa (Lane 1),MCF-7 (Lane 2), Mouse Liver (Lane 3), Rat Brain (Lane 4), using Histone H3 diluted at 1:5,000.
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