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ELK Biotechnology
SKU:EM1035
Lamin B1 Mouse Monoclonal Antibody
Lamin B1 Mouse Monoclonal Antibody
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$248.00 USD
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Lamin B1 Mouse Monoclonal Antibody
Overview
| Product name: | Lamin B1 Mouse mAb |
| Reactivity: | Human, Rat, Mouse |
| Alternative Names: | ADLD, LMB1,LMNB1, MGC111419, OTTHUMP00000159218 |
| Source: | Mouse |
| Applications: | WB, IP |
| Species: | Human, Rat, Mouse |
| Species: | Human |
| Recommended dilutions: | WB 1:2,000-5,000 IP 1:200 |
| Immunogen: | Recombinant Protein |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | 68kDa |
| GeneID(Human): | 4001 |
| Human Swiss-Prot No: | P20700 |
| Cellular localization: | Mitochondrion inner membrane |
| Background: | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. |
Precision
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
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Western blot analysis of 1) HepG2, 2) 293T, 3) Mouse Brain Tissue, 4) Rat Brain Tissue with EM1035 diluted at 1:5,000. -
1、Input: Mouse Brain Tissue Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1035 1:5,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000
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