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ELK Biotechnology
SKU:EM1034
α-tubulin Mouse Monoclonal Antibody
α-tubulin Mouse Monoclonal Antibody
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$248.00 USD
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α-tubulin Mouse Monoclonal Antibody
Overview
| Product name: | α-tubulin Mouse mAb |
| Reactivity: | Human, Rat, Mouse |
| Alternative Names: | alpha tubulin 2, B alpha 1, H2 alpha, Hum a tub 1, TUBA1, TUBA1A,Tubulin alpha 1chain |
| Source: | Mouse |
| Applications: | WB, IHC, IF, IP |
| Species: | Human, Rat, Mouse |
| Species: | Human |
| Recommended dilutions: | WB 1:5,000-10,000 IP 1:200 IF 1:100-200 IHC 1:200-500 |
| Immunogen: | Recombinant Protein |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | 52kDa |
| GeneID(Human): | 7277 |
| Human Swiss-Prot No: | P68366 |
| Cellular localization: | Cytoplasm,Cytoskeleton,Microtubule |
| Background: | Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kiloDaltons. Microtubules are assembled from dimers of α- and β-tubulin. |
Precision
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
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Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue with EM1034 diluted at 1:5,000. -
Immunohistochemical analysis of paraffin-embedded human Colon caricnoma using α tubulin (EM1034)Mouse mAb diluted at 1:500. -
1、Input: Mouse Brain Tissue Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1034 1:5,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5,000 -
IF analysis of Hela with EM1034(Left) and DAPI (Right) diluted at 1:100.
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