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ELK Biotechnology
SKU:EM1032
COX IV Mouse Monoclonal Antibody
COX IV Mouse Monoclonal Antibody
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$248.00 USD
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COX IV Mouse Monoclonal Antibody
Overview
| Product name: | COX IV Mouse mAb |
| Reactivity: | Human, Rat, Mouse |
| Alternative Names: | COX 4, Cox4a, COX4B, COXIV, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, MGC105470, dj857M17.2, Cytochrome c oxidase subunit IV |
| Source: | Mouse |
| Applications: | WB, IF |
| Species: | Human, Rat, Mouse |
| Species: | Human |
| Recommended dilutions: | WB 1:1,000-3,000 IF 1:100-200 |
| Immunogen: | Recombinant Protein |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | 15kDa |
| GeneID(Human): | 1327 |
| Human Swiss-Prot No: | P13073 |
| Cellular localization: | Membrane,Mitochondrion,Mitochondrion inner membrane |
| Background: | The enzyme cytochrome c oxidase or Complex IV, EC 1.9.3.1) is a large transmembrane protein complex found in bacteria and the mitochondrion.It is the last enzyme in the respiratory electron transport chain of mitochondria (or bacteria) located in the mitochondrial (or bacterial) membrane. It receives an electron from each of four cytochrome c molecules, and transfers them to one oxygen molecule, converting molecular oxygen to two molecules of water. In the process, it binds four protons from the inner aqueous phase to make water, and in addition translocates four protons across the membrane, helping to establish a transmembrane difference of proton electrochemical potential that the ATP synthase then uses to synthesize ATP. |
Precision
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
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Western blot analysis of Hela with COX IV Mouse mAb diluted at 1)1:2,000 2)1:5,000 -
Western blot analysis of 1) HepG2, 2) 293T, 3) Rat Heart, 4) Mouse Heart, 5) Mouse Muscle tissue with COX IV Mouse mAb) diluted at 1:3,000. -
Western blot analysis of extracts from Mouse Kidney using COX IV diluted at 1:5,000. -
IF analysis of Hela with EM1032(Left) and DAPI (Right) diluted at 1:100.
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