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ELK Biotechnology

EM1030

PCNA Mouse Monoclonal Antibody

PCNA Mouse Monoclonal Antibody

$248.00 USD
$248.00 USD
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PCNA Mouse Monoclonal Antibody

Overview

Product name: PCNA Mouse mAb
Reactivity: Human, Rat, Mouse
Alternative Names: N/A
Source: Mouse
Applications: WB, IHC
Species: Human, Rat, Mouse
Species: Human
Recommended dilutions: WB 1:5,000 IHC 1:200
Immunogen: Synthetic Peptide
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/ml
Observed band: 29kDa
GeneID（Human）: 5111
Human Swiss-Prot No: P12004
Cellular localization: Nucleus
Background: PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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